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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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ChAP-chip analysis of Hha binding regions in wild type and hnsstpA E. coli cells

ABSTRACT: The analysis of the distribution of Hha binding regions (His-Hha were expressed from pQE plasmid) on E. coli chromosome in E. coli W3110 (wild type) and hns/stpA mutant cells.

ORGANISM(S): Escherichia coli

SUBMITTER: Taku Oshima

PROVIDER: E-MEXP-3813 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Functions of the Hha and YdgT proteins in transcriptional silencing by the nucleoid proteins, H-NS and StpA, in Escherichia coli.

Ueda Takeshi T Takahashi Hiroki H Uyar Ebru E Ishikawa Shu S Ogasawara Naotake N Oshima Taku T

DNA research : an international journal for rapid publication of reports on genes and genomes 20130329 3

The Hha and YdgT proteins are suggested to modulate the expression of horizontally acquired genes by interacting with H-NS and StpA, which play central roles in the transcriptional silencing of such genes. However, it is also possible that Hha/YdgT repress gene expression independently of H-NS/StpA, as we have not fully understood the molecular mechanism through which Hha/YdgT modulate H-NS/StpA activity. To gain further insight into the basic functions of Hha/YdgT, we analysed the impact of hha ...[more]

PMID: 23543115

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Comparative genomic hybridization of Streptococcus suis strains - part 2

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Project description:Genome-wide binding profiles of AbrB and Abh was determined by use of ChAP (chromatin affinity purification)-chip method, modified method of ChIP-chip. Genetic modification was undertaken to abrB and abh genes to translationally fuse 2HC (coding sequence of twelve histidine and chitin binding domain) at the 3' ends to express AbrB-2HC and Abh-2HC proteins respectively.

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2011-09-15 | E-MEXP-3360 | biostudies-arrayexpress

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