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MiRNA profiling of 39 human AML samples

ABSTRACT: 'miRNA expression in 39 human AML samples was analyzed on Sureprint G3 Human miRNA (release 16.0) 8x60K Microarrays from Agilent Technologies. Half the samples represent primary AMLs while the other half represents secondary AMLs. Sample material consisted of cryopreserved bone marrow or blood aspirates. RNA from the samples was purified using the miRNeasy Mini Kit from Qiagen and the sample quality was tested on a Bioanalyser (all sample had RIN values ≥6.0 [mean=8.4]). The samples were labelled with Cy3 and processed according to Agilent''s protocol using vacuum concentration to dry the samples before microarray hybridization. The processed microarrays were scanned at 532 nm using a GenePix 4400A laserscanner at a resolution of 2.5 µm and the GenePix Pro 7.0 software.'

ORGANISM(S): Homo sapiens

SUBMITTER: Christopher Veigaard

PROVIDER: E-MTAB-3627 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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