In-Vitro Cell Adhesion Assay

ABSTRACT: Cigarette smoke (CS) has been reported to affect the adhesion of monocytes to endothelial cells, a critical step in atherogenesis. Using an in vitro adhesion assay together with innovative computational systems biology approaches to analyze omics data, our study aimed at investigating CS-induced mechanisms by which monocyte-endothelial cell adhesion is promoted. Disease-relevant primary human coronary artery endothelial cells (HCAECs) were treated for 4 hours with (1) conditioned media of human monocytic Mono Mac-6 (MM6) cells preincubated with low or high concentrations of aqueous CS extract (sbPBS) from reference cigarette 3R4F for 2 hours (indirect treatment, I), (2) unconditioned media similarly prepared, however without MM6 cells (direct treatment, D), or (3) freshly generated sbPBS (

INSTRUMENT(S): Qiagen-Qiacube, Affymetrix GeneChip Scanner 3000 7G

ORGANISM(S): Homo sapiens

SUBMITTER: Sam Ansari 

PROVIDER: E-MTAB-3644 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress