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In vitro systems toxicology-based assessment of the potential modified risk tobacco product CHTP1.2 for vascular inflammation- and cytotoxicity-associated mechanisms promoting adhesion of monocytic cells to human coronary arterial endothelial cells.


ABSTRACT: Cigarette smoke causes diseases such as cardiovascular disorders. Heating tobacco, instead of burning, reduces consistently the amount of toxic compounds and may exert a reduced impact on cardiovascular health compared to cigarettes. Aqueous extract from the aerosol of a potential modified risk tobacco product, the carbon-heated tobacco product (CHTP) 1.2 was assessed in vitro for its impact, compared with a reference cigarette (3R4F), on a key mechanism of atherosclerosis, the adhesion of monocytic cells to artery endothelial cells. Human coronary arterial endothelial cells (HCAECs) were treated for 4h with conditioned media from monocytic Mono Mac-6 (MM6) cells exposed to the aqueous aerosol/smoke extracts for 2h (Indirect exposure) or directly with those extracts freshly generated (Fresh Direct exposure). Using a previously established in vitro adhesion assay design, MM6-HCAEC adhesion combined with inflammatory, oxidative stress, cytotoxicity and cell death markers was measured. The 3R4F extract promoted the adhesion of MM6 cells to HCAECs via distinct inflammatory- and cytotoxicity-driven mechanisms. In conclusions, our systems toxicology study demonstrated that approximately 10-15-fold higher concentrations of the CHTP1.2 aerosol extract were needed to elicit similar effects as the 3R4F smoke extract on cardiovascular disease-relevant inflammation and cytotoxicity-related mechanisms and markers investigated in vitro.

ORGANISM(S): Homo sapiens

SUBMITTER: Alain Sewer 

PROVIDER: E-MTAB-6721 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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In vitro systems toxicology-based assessment of the potential modified risk tobacco product CHTP 1.2 for vascular inflammation- and cytotoxicity-associated mechanisms promoting adhesion of monocytic cells to human coronary arterial endothelial cells.

Poussin Carine C   Laurent Alexandra A   Kondylis Athanasios A   Marescotti Diego D   van der Toorn Marco M   Guedj Emmanuel E   Goedertier Didier D   Acali Stefano S   Pak Claudius C   Dulize Rémi R   Baumer Karine K   Peric Dariusz D   Maluenda Elodie E   Bornand David D   Suarez Ignacio Gonzalez IG   Schlage Walter K WK   Ivanov Nikolai V NV   Peitsch Manuel C MC   Hoeng Julia J  

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 20180717


Cigarette smoking causes cardiovascular diseases. Heating tobacco instead of burning it reduces the amount of toxic compounds in the aerosol and may exert a reduced impact on health compared with cigarette smoke. Aqueous extract from the aerosol of a potential modified risk tobacco product, the Carbon Heated Tobacco Product (CHTP) 1.2, was compared in vitro with aqueous extract from the smoke of a 3R4F reference cigarette for its impact on the adhesion of monocytic cells to artery endothelial ce  ...[more]

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