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Analysis of gene expression changes in response to hypoxia and/or cisplatin in A2780 and A2780cis cells

ABSTRACT: The aim of this experiment was to evaluate the changes in gene expression in response to hypoxia and/or cisplatin in an ovarian cancer cell line model. A2780 (cisplatin-sensitive) and A2780cis (cisplatin-resistant) cell lines were treated with cisplatin in normoxia or hypoxia (0.5% O2) for 72 hours. RNA was extracted from three independent biological replicates for each condition: A2780 (normoxia, untreated); A2780 (hypoxia, untreated); A2780 (normoxia, cisplatin); A2780cis (hypoxia, cisplatin), A2780cis (normoxia, untreated); A2780cis (hypoxia, untreated); A2780cis (normoxia, cisplatin); A2780cis (hypoxia, cisplatin) and interrogated on Affymetrix Human Gene ST 1.0 arrays.

INSTRUMENT(S): Genechip scanner, INVIVO2 hypoxia chamber

ORGANISM(S): Homo sapiens

SUBMITTER: Lynda McEvoy

PROVIDER: E-MTAB-3645 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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