Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

50 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of poly-A enriched total RNA of brain, liver, kidney, heart and skeletal muscle samples from mouse

ABSTRACT: This experiment contains the Mus musculus subset of data from the experiment E-GEOD-41338 (http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41338/). mRNA profiles of several organs (brain, liver, kidney, heart, skeletal muscle) in multiple vertebrate species (mouse, chicken, lizard, frog, pufferfish) were generated by deep sequencing using Illumina HiSeq to better understand how species with similar repertoires of protein-coding genes differ so markedly at the phenotypic level.

ORGANISM(S): Mus musculus

SUBMITTER: Nuno Barbosa-Morais

PROVIDER: E-MTAB-3725 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

The evolutionary landscape of alternative splicing in vertebrate species.

Barbosa-Morais Nuno L NL Irimia Manuel M Pan Qun Q Xiong Hui Y HY Gueroussov Serge S Lee Leo J LJ Slobodeniuc Valentina V Kutter Claudia C Watt Stephen S Colak Recep R Kim TaeHyung T Misquitta-Ali Christine M CM Wilson Michael D MD Kim Philip M PM Odom Duncan T DT Frey Brendan J BJ Blencowe Benjamin J BJ

Science (New York, N.Y.) 20121201 6114

How species with similar repertoires of protein-coding genes differ so markedly at the phenotypic level is poorly understood. By comparing organ transcriptomes from vertebrate species spanning ~350 million years of evolution, we observed significant differences in alternative splicing complexity between vertebrate lineages, with the highest complexity in primates. Within 6 million years, the splicing profiles of physiologically equivalent organs diverged such that they are more strongly related ...[more]

PMID: 23258890

Similar Datasets

RNA-seq of poly-A enriched total RNA of brain, liver, kidney, heart and skeletal muscle samples from chicken

Project description:This experiment contains the Gallus gallus subset of data from the experiment E-GEOD-41338 (http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41338/). mRNA profiles of several organs (brain, liver, kidney, heart, skeletal muscle) in multiple vertebrate species (mouse, chicken, lizard, frog, pufferfish) were generated by deep sequencing using Illumina HiSeq to better understand how species with similar repertoires of protein-coding genes differ so markedly at the phenotypic level.

2012-12-22 | E-MTAB-3724 | biostudies-arrayexpress

RNA-seq of poly-A enriched total RNA of brain, liver, kidney, heart and skeletal muscle samples from frog

Project description:This experiment contains the Xenopus tropicalis subset of data from the experiment E-GEOD-41338 (http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41338/). mRNA profiles of several organs (brain, liver, kidney, heart, skeletal muscle) in multiple vertebrate species (mouse, chicken, lizard, frog, pufferfish) were generated by deep sequencing using Illumina HiSeq to better understand how species with similar repertoires of protein-coding genes differ so markedly at the phenotypic level.

2012-12-22 | E-MTAB-3726 | biostudies-arrayexpress

RNA-seq of poly-A enriched total RNA of brain, liver, kidney, heart and skeletal muscle samples from lizard

Project description:This experiment contains the Anolis carolinensis subset of data from the experiment E-GEOD-41338 (http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41338/). mRNA profiles of several organs (brain, liver, kidney, heart, skeletal muscle) in multiple vertebrate species (mouse, chicken, lizard, frog, pufferfish) were generated by deep sequencing using Illumina HiSeq to better understand how species with similar repertoires of protein-coding genes differ so markedly at the phenotypic level.

2012-12-22 | E-MTAB-3727 | biostudies-arrayexpress

RNA-seq of poly-A enriched total RNA of brain, liver, kidney, heart and skeletal muscle samples from pufferfish

Project description:This experiment contains the Tetraodon nigroviridis subset of data from the experiment E-GEOD-41338 (http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41338/). mRNA profiles of several organs (brain, liver, kidney, heart, skeletal muscle) in multiple vertebrate species (mouse, chicken, lizard, frog, pufferfish) were generated by deep sequencing using Illumina HiSeq to better understand how species with similar repertoires of protein-coding genes differ so markedly at the phenotypic level.

2012-12-22 | E-MTAB-3728 | biostudies-arrayexpress

Muscleblind-like proteins regulate embryonic stem cell-specific alternative splicing and reprogramming I

Project description:Previous investigations of the core gene regulatory circuitry that controls embryonic stem cell (ESC) pluripotency have largely focused on the roles of transcription, chromatin and non- coding RNA regulators. Alternative splicing (AS) represents a widely acting mode of gene regulation, yet its role in the regulation of ESC pluripotency and differentiation is poorly understood. Here, we identify the Muscleblind-like RNA binding proteins, MBNL1 and MBNL2, as conserved and direct negative regulators of a large program of AS events that are differentially regulated between ESCs and other cell types. Knockdown of MBNL proteins in differentiated cells causes switching to an ESC-like AS pattern for at least half of these AS events. Among the events is an ESC-specific AS switch in the forkhead family transcription factor FOXP1 that controls pluripotency. Consistent with a central and negative regulatory role for MBNL proteins in pluripotency, their knockdown significantly enhances the expression of key pluripotency genes and the formation of induced pluripotent stem cells (iPSCs) during somatic cell reprogramming. mRNA profiles of various embryonic stem cells, tissues and cell lines from human and mouse using high-throughput sequencing data and the role of MBNL proteins in regulation of ESC-differential alternative splicing

2013-06-26 | E-GEOD-45503 | biostudies-arrayexpress

Muscleblind-like proteins regulate embryonic stem cell-specific alternative splicing and reprogramming II

2013-06-26 | E-GEOD-45504 | biostudies-arrayexpress

Essential roles for the splicing regulator nSR100/SRRM4 during nervous system development

Project description:Alternative splicing (AS) generates vast transcriptomic complexity in the vertebrate nervous system. However, the extent to which trans-acting splicing regulators and their target AS regulatory networks contribute to nervous system development is not completely understood. To address these questions, we have generated mice lacking the vertebrate- and neural-specific Ser/Arg-repeat related protein of 100 kDa (nSR100/SRRM4). Loss of nSR100 impairs development of the central and peripheral nervous systems, in part by disrupting neurite outgrowth, cortical layering in the forebrain, and axon guidance in the corpus callosum. Accompanying these developmental defects are widespread changes in AS that primarily result in shifts to non-neural patterns for different classes of splicing events. The main component of the altered AS program comprises 3-27 nucleotide neural microexons, an emerging class of highly conserved alternative splicing event associated with the regulation of protein interaction networks in developing neurons and neurological disorders. Remarkably, inclusion of a 6-nucleotide nSR100-activated microexon in Unc13b transcripts is sufficient to rescue a neuritogenesis defect in nSR100 mutant primary neurons. These results thus reveal critical in vivo neurodevelopmental functions of nSR100, and they further link these functions to a conserved program of neural microexon splicing. mRNA profiles of mouse control or nSR100/Srrm4 KO cortex or hippocampus using high-throughput sequencing data.

2015-04-14 | E-GEOD-65818 | biostudies-arrayexpress

Conserved functional antagonism between CELF and MBNL proteins regulates stem cell-specific alternative splicing and regeneration in planarians

Project description:Understanding gene regulation in stem cells is key to understanding stem cell biology. Contrary to transcriptional regulation the function of alternative splicing (AS) in stem cells is poorly understood. Here we study function and mechanisms of AS in a powerful in vivo model for stem cell biology, the planarian S. mediterranea. Knockdown of AS factors, computational analyses of massive RNA-sequencing data, phenotyping, and biochemical binding assays revealed a stem cell specific AS program comprising hundreds of alternative exons, intron retention events, and microexons. The conserved AS factors CELF/MBNL are main regulators of this program. We show that they antagonize each other by directly promoting or repressing stem cell specific AS events. Knockdown of CELF/MBNL leads to defective regeneration by affecting self-renewal and differentiation, respectively. Thus, AS is of key importance for stem cell regulation in vivo and antagonistic regulation by CELF/MBNL is likely an ancestral feature of animal stem cells. mRNA profiles of various samples of planarian cells, including control and knockdowns of key splicing factors in whole worms, and FACS-purified fractions

2016-08-01 | E-GEOD-75594 | biostudies-arrayexpress

RNA-seq of poly-A enriched total RNA of brain, liver, kidney, heart and skeletal muscle samples from 5 vertebrate species: mouse, chicken, frog, lizard and pufferfish

Project description:How species with similar repertoires of protein coding genes differ so dramatically at the phenotypic level is poorly understood. From comparing the transcriptomes of multiple organs from vertebrate species spanning ~350 million years of evolution, we observe significant differences in alternative splicing complexity between the main vertebrate lineages, with the highest complexity in the primate lineage. Moreover, within as little as six million years, the splicing profiles of physiologically-equivalent organs have diverged to the extent that they are more strongly related to the identity of a species than they are to organ type. Most vertebrate species-specific splicing patterns are governed by the highly variable use of a largely conserved cis-regulatory code. However, a smaller number of pronounced species-dependent splicing changes are predicted to remodel interactions involving factors acting at multiple steps in gene regulation. These events are expected to further contribute to the dramatic diversification of alternative splicing as well as to other gene regulatory changes that contribute to phenotypic differences among vertebrate species. mRNA profiles of several organs (brain, liver, kidney, heart, skeletal muscle) in multiple vertebrate species (mouse, chicken, lizard, frog, pufferfish) generated by deep sequencing using Illumina HiSeq

2012-12-22 | E-GEOD-41338 | biostudies-arrayexpress

Deep Biosphere - Beatrix 326 BH2 (270712)

Project description:This project aims to investigate the metabolic pathways expressed by the active microbial community occurring at the deep continental subsurface. Subsurface chemoLithoautotrophic Microbial Ecosystems (SLiMEs) under oligotrophic conditions are supported by H2; however, the overall ecological trophic structures of these communities are poorly understood. Some deep, fluid-filled fractures in the Witwatersrand Basin, South Africa appear to support inverted trophic pyramids wherein methanogens contributing <5% of the total DNA apparently produce CH4 that supports the rest of the community. Here we show the active metabolic relationships of one such trophic structure by combining metatranscriptomic assemblies, metaproteomic and stable isotopic data, and thermodynamic modeling. Four autotrophic β-proteobacteria genera that are capable of oxidizing sulfur by denitrification dominate. They co-occur with sulfate reducers, anaerobic methane oxidizers and methanogens, which each comprises <5% of the total community. Defining trophic levels of microbial chemolithoautotrophs by the number of transfers from the initial abiotic H2-driven CO2 fixation, we propose a top-down cascade influence of the metabolic consumers that enhances the fitness of the metabolic producers to explain the inverted biomass pyramid of a multitrophic SLiME. Symbiotic partnerships are pivotal in the deep biosphere on and potentially beyond the Earth.

2018-10-27 | PXD004634 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data