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Microarray analysis of newborn mice carrying a mutation in miR-96, compared to wildtype sex-matched littermate controls.

ABSTRACT: The diminuendo mouse carries a mutation in the seed region of miR-96. Adult homozygotes are deaf and display vestibular defects, heterozygotes have rapidly progressive hearing loss. Microarray analysis of four day old homozygotes showed multiple transcripts were misregulated. Because the effect of the diminuendo mutation appears to be a retardation of development, we carried out this microarray on newborn homozygote and wildtype mice to examine the transcriptome at a younger age.

INSTRUMENT(S): Beadarray reader

ORGANISM(S): Mus musculus

SUBMITTER: Morag Lewis

PROVIDER: E-MTAB-3735 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Exploring regulatory networks of miR-96 in the developing inner ear.

Lewis Morag A MA Buniello Annalisa A Hilton Jennifer M JM Zhu Fei F Zhang William I WI Evans Stephanie S van Dongen Stijn S Enright Anton J AJ Steel Karen P KP

Scientific reports 20160318

Mutations in the microRNA Mir96 cause deafness in mice and humans. In the diminuendo mouse, which carries a single base pair change in the seed region of miR-96, the sensory hair cells crucial for hearing fail to develop fully and retain immature characteristics, suggesting that miR-96 is important for coordinating hair cell maturation. Our previous transcriptional analyses show that many genes are misregulated in the diminuendo inner ear and we report here further misregulated genes. We have ch ...[more]

PMID: 26988146

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Analysis of RNA associated with the RISC complex (Ago2) in four day old mice carrying a mutation in miR-96

Project description:The diminuendo mouse carries a mutation in the seed region of miR-96. Homozygotes are deaf and exhibit vestibular dysfunction, heterozygotes display rapidly progressive hearing loss. Some targets of miR-96 are known, but it is likely that many still remain to be discovered. MicroRNAs function by recruiting the RISC complex to their mRNA targets, so one way to examine the mRNAs being controlled by microRNAs in a cell is to immunoprecipitate Ago2, one of the RISC proteins, and extract the RNA bound to it. This should detect all the mRNAs being targetted by microRNAs in the tissue. We extracted mRNA bound to Ago2 from the organs of Corti and olfactory bulbs of diminuendo homozygote and wildtype littermates at four days old, and carried out microarray analysis.

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