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IGF2 shRNA knockdown in the colorectal cancer (CRC) cell line SW480

ABSTRACT: In order to identify gene expression changes associated with the deregulated of IGF2, Doxycylin inducible empty vector and IGF2 shRNA permanent cell lines were generated by lentivral transduction in the colorectal cancer (CRC) cell line SW480 and selected with puromycin. For microarray analysis, both cell lines were plated at 3 x 105 cells in 6 well plates using RPMI plus 10% FCS and penicillin/ streptomycin, either without/ with 50 ng/ ml doxycycline at the time of plating. The respective cell lines were harvested at day 1, 3, and 5. Three experiments were performed for each time point and condition and were separately analyzed. The samples were analyzed on Illumina human HT-12 v 4 expression bead chips. The microarray data was processed and normalized using the lumi pipline.

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Rogers A.

PROVIDER: E-MTAB-3786 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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