Project description:Two lines of Trypanosoma brucei were isolated from cattle in Uganda. The DNA is from parasites that had undergone two rodent passages.
Project description:Fresh T. b. brucei isolates MAK65 and MAK98 (see E-MTAB-9320) were cultured in vitro for various times then the genomic DNA was sequenced.
Project description:Human African Trypanosomiasis (HAT) is a disease of major economic importance in Sub-Saharan Africa. In eastern and southern Africa. Here we analysed clinical isolates of T brucei rhodensiense, resistant to suramin by shotgun proteomics . And identified parasite proteins whose expression is associated with resistance to suramin.
Project description:Since the initial publication of the trypanosomatid genomes, curation has been ongoing. Here we apply the technique of ribosome profiling to Trypanosoma brucei, identifying 223 new coding regions by virtue of ribosome occupancy in the corresponding transcripts. A small number of these putative genes correspond to extra copies of previously annotated genes but 85% are novel. The median size of these novels CDSs is small (74 aa) indicating that past annotation work has excelled at detecting large CDSs. Of the unique CDSs discovered here, over half have candidate orthologues in other trypanosomatid genomes, most of which were not yet annotated as genes. Still, approximately one-third of the new CDSs were found only in T. brucei subspecies. When combined with RNA-seq and spliced leader mapping, we were able to definitively revise the start sites for 430 CDSs as compared to the current gene models. Such data also allowed us to use a structured approach to eliminate 701 putative genes as protein-coding. Finally, the data pointed to several regions of the genome that had sequence errors that altered coding region boundaries. Ribosome profiling and mRNA libraries were constructed in triplicate from in vitro PCF and in vivo BF lifestages of theT. brucei Treu927 and in vitro T. brucei Lister427, to evaluate role of translational gene regulation