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Genetic variability exceeds cell-type memory and determines iPSCs differentiation potential-implications for biobanking

ABSTRACT: To address the issue of retention of somatic cell memory in iPSCs we compared methylation profiles of genetically matched human iPSCs derived from fibroblasts and blood. We were using the all-in-one type footprint free SeVdp-iPS system, for generation of uniform iPSC lines. Our results show that iPSC lines derived from the same donor are highly similar to each other. Experimental design: methylation profiles were assayed by Reduced Representation Bisulfite Sequencing (RRBS) for fibroblast-derived iPSC (N=8) and blood-derived iPSC (N=10) as well as isogenic parental fibroblasts and peripheral blood mononuclear cells (N=4 different donors: T14; T42; T53; T55). H9 human embryonic stem cell lines was used as control.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Cristina Valensisi

PROVIDER: E-MTAB-3859 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genetic variability exceeds cell-type memory and determines iPSCs differentiation potential-implications for biobanking

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