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RNA-Seq data of 16 strains of saccharomyces cerevisiae produced after knockout of all combination of HIS3, LEU2, URA3 and MET15 genes

ABSTRACT: RNA-Seq data of 16 strains of saccharomyces cerevisiae produced after knockout of all combination of HIS3, LEU2, URA3 and MET15 genes

PROVIDER: PRJEB11515 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1095160_1.fastq.gz	Fastqsanger.gz
	ERR1095160_2.fastq.gz	Fastqsanger.gz
	ERR1095170_1.fastq.gz	Fastqsanger.gz
	ERR1095170_2.fastq.gz	Fastqsanger.gz
	ERR1095180_1.fastq.gz	Fastqsanger.gz

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RNA-Seq data of 16 strains of saccharomyces cerevisiae produced after knockout of all combination of HIS3, LEU2, URA3 and MET15 genes

Project description:In this study we have deleted four metabolic genes (HIS3, LEU2, URA3 and MET15) in their sixteen possible combinations. These strains are following: knockout of HIS3, LEU2, URA3, MET15; knockout of HIS3, LEU2, MET15; knockout of HIS3, URA3, MET15; knockout of LEU2, URA3, MET15; knockout of HIS3, LEU2, URA3; knockout of HIS3, MET15; knockout of LEU2, MET15; knockout of URA3, MET15; knockout of HIS3, LEU2; knockout of HIS3, URA3; knockout of LEU2, URA3; knockout of MET15, knockout of HIS3; knockout of LEU2; knockout of URA3 and prototrophic strain. We grew the 16 strains in synthetic complete media (SC), and sampled for transcriptomincs in triplicates at mid-exponential phase (OD value of 0.8).

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