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RNA-seq analysis to investigate the downstream genes of EXB1 in Arabidopsis

ABSTRACT: Arabidopsis EXCESSIVE BRANCHES 1 (EXB1) affects both axillary meristem (AM) initiation and bud activities. To investigate the downstream genes of EXB1, the construct 4EnhpEXB1-EXB1GR in which EXB1 was fused to the sequence encoding the steroid-binding domain of the rat glucocorticoid receptor (GR) was constructed and EXB1 was driven by four CaMV 35S enhancers and EXB1 promoter. We performed RNA-seq analysis on the tissues around leaf axils of 14-day-old 4EnhpEXB1-EXB1GR-13 transgenic lines treated with or without DEX. The RNA-seq results showed that 3114 genes were differentially expressed, with 1015 up-regulated and 2099 down-regulated. Among these genes, three MYB family transcription factor genes, i.e., RAX1, RAX2 and RAX3, known to regulate axillary meristem initiation were identified suggesting that RAX1, RAX2 and RAX3 are probably downstream genes of EXB1.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Genji Qin

PROVIDER: E-MTAB-4002 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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