Intestinal epithelial HuR balances counteracting RNA networks to protect against intestinal inflammation and cancer. PART 2 Differentiality in mRNA expression between Control mice and mice overexpressing Elavl1.
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ABSTRACT: Elavl1/HuR is a ubiquitous and conserved RNA-binding protein that binds to a U-rich RNA motif that shuttles between nucleus and cytoplasm. In epithelia, the elevated expression of HuR assumingly promotes degeneration and cancer suggesting that its generic suppression may provide clinical benefits. In this study we focused on biological and clinical functions of HuR in intestinal epithelial cells and we presented evidence that changes in HuR levels induce polarized distortions in these cells to support different pathologic outcomes. Here we study the differentiality in mRNA abundance between Control mice and mice overexpressing Elavl1 (TgATF-HuR). Control and TgATF-HuR mice were treated with Dimethylhydrazine (DMH)/Dextran Sodium Sulphate (DSS) for 60 days and tumors where dissected from large intestines; those with sizes between 10-15mm2 were pooled to generate samples with 4 tumors/sample and snap frozen. Three samples per genotype were used either for RIP analyses or total RNA extraction. Isolated RNA was used for microarray or qRT-PCR analyses.
INSTRUMENT(S): Scann Array Express
ORGANISM(S): Mus musculus
SUBMITTER: Dimitris Kontoyiannis
PROVIDER: E-MTAB-4017 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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