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Transcriptome profiling of human anaplastic meningioma IOMM-Lee cells following silencing of HuR (Human antigen R) gene expression by siRNA

ABSTRACT: The human anaplastic meningioma IOMM-Lee cells were cultured in Dulbeccoâs modified Eagleâs medium (Life Technologies, Carlsbad, California, USA) supplemented with 10% fetal bovine serum, 100 U/ml penicillin, and 100 mg/ml streptomycin, at 37Â°C in 20% O2 (5% CO2). HuR silencing was achieved by transfecting cells in a 96 well-plate with 500 Âµl per well of culture medium containing 10 Âµl of siRNA (Silencer Select siRNA ELAVL1 s4608; Ambion, Life Technologies; 1 Âµmol/L),1 ÂµL of RNAiMAX lipofectamin and 89 ÂµL of opti-MEM (Life Technologies). Negative control cells were obtained under the same conditions using the Silencer Select Negative Control siRNA#1 (Ambion, Life Technologies). All assays were performed in sextaplicates.

ORGANISM(S): Homo sapiens

SUBMITTER: Laurent Vallar

PROVIDER: E-MTAB-4999 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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