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The role of androgen signalling in human benign prostatic hyperplasia.

ABSTRACT: Treatment of prostate stroma with dihydrotestosterone to examine differential gene expression. Isolation of androgen receptor fraction of stroma using cd49a antibody, RNA extraction, one cycle cDNA synthesis and IVT labelling standard protocols same for each microarray

ORGANISM(S): Homo sapiens

SUBMITTER: paul anthony berry

PROVIDER: E-MTAB-402 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The role of androgen signalling in human benign prostatic hyperplasia.

Project description:Treatment of prostate stroma with dihydrotestosterone to examine differential gene expression. Isolation of androgen receptor fraction of stroma using cd49a antibody, RNA extraction, one cycle cDNA synthesis and IVT labelling standard protocols same for each microarray

2011-10-01 | E-MTAB-600 | biostudies-arrayexpress

Transcription profiling of human prostate cancer cells, normal epithelial prostatic cells and stroma cells from patients in placebo, selenium, vitamin E or selenium and vitamin E treatment groups

Project description:85 radical prostatectomy specimens (where 16 samples are in Placebo group (PL), 15 are in Selenium group (SE), 25 are in Vitamin E group (VE) and 27 are in Vitamin E & Selenium group (VS.Treatment groups: l-selenomethionine, 400 ug + placebo (vitamin E); vitamin E, 400 IU + placebo (l-selenomethionine); l-selenomethionine, 400 ug + vitamin E, 400 IU; placebos) were subjected to laser capture microdissection to isolate prostate cancer cells, normal epithelial prostatic cells, or stroma cells. RNA extraction and amplification were performed. Transcription profiling was conducted using the HG-U133A Affymetrix arrays.

2008-07-01 | E-MEXP-1327 | biostudies-arrayexpress

Transcription profiling of human fibroblasts induced to transdifferentiate in response to TGF-b

Project description:Since the human prostate does not undergo replicative senescence but rather undergoes transdifferentiation with increasing age, we investigated the molecular profile of prostate fibroblasts induced to transdifferentiate in response to TGF-b1.

2010-05-04 | E-MEXP-2167 | biostudies-arrayexpress

Transcription profiling by array of human stromal cells cultured with or without 3D BPH-1 prostate epithelial cells

Project description:BPH-1 prostate epithelial cells when cultured in Matrigel form 3D acini which closely recapitulate the tissue architecture of the prostate. The presence of primary stroma increases epithelial adhesions and causes changes in morphology. To identify the stromal signals which control actin/cadherin dynamics in epithelial cells we performed a microarray analysis on the stroma using Affymetrix U133 plus 2.0 arrays. Primary stroma from 7 different patients were cultured with (PSE) or without (PS) 3D BPH-1 epithelial acini to determine the gene expression changes within the stroma. The RNA from the Primary epithelia and stromal cultures were used at passages 1 or 1-3, respectively.

2010-06-09 | E-MEXP-2657 | biostudies-arrayexpress

Cells Comprising the Prostate Cancer Microenvironment Lack Recurrent Clonal Somatic Genomic Aberrations

Project description:Prostate cancer-associated stroma (CAS) plays an active role in malignant transformation, tumor progression, and metastasis. Molecular analyses of CAS have demonstrated significant changes in gene expression; however, conflicting evidence exists on whether genomic alterations in benign cells comprising the tumor microenvironment (TME) underlie gene expression changes and oncogenic phenotypes. This study evaluates the nuclear and mitochondrial DNA integrity of prostate carcinoma cells, CAS, matched benign epithelium and benign epithelium-associated stroma by whole genome copy number analyses, targeted sequencing of TP53, and fluorescence in situ hybridization. Comparative genomic hybridization (aCGH) of CAS revealed a copy-neutral diploid genome with only rare and small somatic copy number aberrations (SCNAs). In contrast, several expected recurrent SCNAs were evident in the adjacent prostate carcinoma cells, including gains at 3q, 7p, and 8q, and losses at 8p and 10q. No somatic TP53 mutations were observed in CAS. Mitochondrial DNA (mtDNA) extracted from carcinoma cells and stroma identified 23 somatic mtDNA mutations in neoplastic epithelial cells but only one mutation in stroma. Finally, genomic analyses identified no SCNAs, no loss of heterozygosity (LOH) or copy-neutral LOH in cultured cancer-associated fibroblasts (CAFs), which are known to promote prostate cancer progression in vivo. Agilent whole human genome aCGH oligonucleotide microarrays were used to assess copy number aberrations in 79 specimens from 20 patients as well as two pairs of cancer-associated fibroblasts (CAF) and benign/normal associated fibroblasts (NAF). 3 CAF/NAF sample pairs were also assessed for DNA copy number aberrations and copy-neutral LOH using the Infinium HumanOmniExpressExome BeadChip Kit.

2016-02-12 | E-GEOD-76456 | biostudies-arrayexpress

LC-MS/MS of chloroplasts from WT plants and fractionated the proteins into the membrane and stroma fractions

Project description:To confirm the localization of GCSC1 to chloroplast vesicle rather than in the stroma, we purified chloroplasts from WT plants and fractionated the proteins into the membrane and stroma fractions. Quantification of proteins by liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed that GCSC1 was present only in the membrane fractions, co-existing with several chloroplast membrane proteins.

2024-09-26 | PXD056230 | Pride

Transcription profiling of human primary prostate epithelial and stromal cells exposed to 4-methylsulphinylbutyl and 3-methylsulphinypropyl isothiocyanates

Project description:BACKGROUND: Broccoli consumption has been associated with a reduction in risk of prostate cancer. Isothiocyanates (ITC) derived from glucosinolates that accumulate in broccoli are potential dietary compounds that may mediate these health effects. Sulforaphane (SF, 4-methylsulphinylbutyl ITC) accumulates in heading broccoli (calabrese) and iberin (IB, 3-methylsulphinypropyl ITC) accumulates in sprouting broccoli. While, there are many studies regarding the biological activity of SF, there are few studies associated with IB. Moreover, the majority of studies have been undertaken with transformed cells, usually epithelial in origin, that have cancerous phenotypes. <br>METHOD: Primary epithelial and stromal cells were derived from benign prostatic hyperplasia tissue. Affymetrix U133 Plus 2.0 whole genome arrays were used to quantify global gene expression changes in these cells, following exposure to physiologically appropriate concentrations of SF and IB. Ontology and pathway analyses were used to interpret results. Changes in expression of a subset of genes were confirmed by RT PCR.<br>RESULTS: Global gene expression profiling identified epithelial and stromal specific gene expression profiles. IB and SF induced different changes in gene expression in both epithelial and stromal cells but these changes were associated with similar functions<br>CONCLUSION: This data suggests that IB and SF both induce genes associated with cancer prevention, and IB should be further investigated as a potential chemo-preventative target. <br>

2009-06-05 | E-MEXP-2034 | biostudies-arrayexpress

Activation of the P-TEFb Complex by Lethal Prostate Cancer-Associated Enhancer RNAs

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-02-12 | E-GEOD-55032 | biostudies-arrayexpress

Transcription profiling of human prostate samples from normal donors and prostate cancer patients

Project description:This SuperSeries is composed of the following subset Series:; GSE6604: Expression data from Normal Prostate Tissue free of any pathological alteration; GSE6605: Expression data from Metastatic Prostate Tumor; GSE6606: Expression data from Primary Prostate Tumor; GSE6608: Expression data from Normal Prostate Tissue Adjacent to Tumor Experiment Overall Design: Refer to individual Series

2008-04-06 | E-GEOD-6919 | biostudies-arrayexpress

TMPRSS2-ERG, HDACs and EZH2 are involved in an AR-centric transcriptional circuitry that calibrates androgenic response for prostate cancer progression

Project description:This SuperSeries is composed of the following subset Series: GSE28948: TMPRSS2-ERG, HDACs and EZH2 are involved in an AR-centric transcriptional circuitry that calibrates androgenic response for prostate cancer progression (gene expression data) GSE28950: TMPRSS2-ERG, HDACs and EZH2 are involved in an AR-centric transcriptional circuitry that calibrates androgenic response for prostate cancer progression (ChIP-Seq data) GSE35540: TMPRSS2-ERG, HDACs and EZH2 are involved in an AR centric transcriptional circuitry that calibrates androgenic response for prostate cancer progression (gene expression after ERG KD) Refer to individual Series

2012-04-26 | E-GEOD-28951 | biostudies-arrayexpress

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