Dataset Information
Continuous CRISPR spacer acquisition in STSV2-infected crenarchaeon Sulfolobus islandicus REY15A over a 6-day period
Ontology highlight
ABSTRACT:
INSTRUMENT(S): TRIzol (Sigma Aldrich), Illumina MiSeq, ScriptSeq complete kit Low input (Bacteria) (Epicentre), Illumina HiSeq 2000
ORGANISM(S): Sulfolobus islandicus
SUBMITTER: Carlos Len-Sobrino
PROVIDER: E-MTAB-4027 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Transcriptome changes in STSV2-infected Sulfolobus islandicus REY15A undergoing continuous CRISPR spacer acquisition.
Molecular microbiology 20151127 4
A transcriptome study was performed on Sulfolobus islandicus REY15A actively undergoing CRISPR spacer acquisition from the crenarchaeal monocaudavirus STSV2 in rich and basal media over a 6 day period. Spacer acquisition preceded strong host growth retardation, altered transcriptional activity of four different CRISPR-Cas modules and changes in viral copy numbers, and with significant differences in the two media. Transcript levels of proteins involved in the cell cycle were reduced, whereas tho ...[more]
