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Type V-A Spacer Acquisition

ABSTRACT: Spacer Acquisition Type V-A CRISPR-Cas system Francisella novicida

PROVIDER: PRJEB34159 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3500113.fastq.gz	Fastqsanger.gz
	ERR3500114.fastq.gz	Fastqsanger.gz
	ERR3500115.fastq.gz	Fastqsanger.gz
	ERR3500116.fastq.gz	Fastqsanger.gz
	ERR3500117.fastq.gz	Fastqsanger.gz

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Project description:A whole transcriptome study was performed on Sulfolobus islandicus REY15A actively undergoing CRISPR spacer acquisition from the crenarchaeal monocaudavirus STSV2 in rich (TYS) and basal (SCV) media over a 6 day period. Spacer acquisition preceded strong host growth retardation, and changes in viral transcript abundance and virus copy numbers showed significant differences between the two media. Results showed that rich medium favoured CRISPR-Cas immunity generation.

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Project description:V. cholerae A50 has a functional CRISPR-cas system with a conserved boxA sequence. Plasmids harboring protospacers that are perfect targets for each spacer of the array are introduced into wt and boxA mutant V. cholerae. After a period of growth without selection, cells are collected and the protospacer plasmids are sequenced in a high throughput manner.

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