Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression profiling of two Alexandrium genotypes as response to waterborne cues from grazing Polykrikos

ABSTRACT: Two Alexandrium fundyense (former tamarense) genotypes with different lytic capacties were analyzed with respect to their response towards co-occuring protistan grazers (Polykrikos) grazing on conspecifics. One Alexandrium genotype was able to lyse the grazer whereas the other genotype completely lacks this ability. The difference in their genotypes resulted in the creation of different niches and in difference in their gene expression response.

ORGANISM(S): Alexandrium fundyense

SUBMITTER: Sylke Wohlrab

PROVIDER: E-MTAB-4060 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Similar Datasets

Project description:Neuroblastoma (NB) is the most frequent extracranial solid tumour of childhood. Clinical courses are highly variable, ranging from spontaneous regression/maturation to rapid progression despite intensive multimodal therapy. The estimation of 5-year event free survival in high-risk patients of only about 40 % stresses an importance of novel therapeutic strategies. A number of iron chelators have demonstrated marked in vitro and in vivo anti-tumor activity and are currently being developed as novel anti-cancer agents. Therefore, the potential antitumor effect of iron chelators in NB cancer was investigated. Among the compounds tested, ciclopirox olamine (CPX) was shown to be one of the most effective intracellular iron chelators in NB cells. To unveil the molecular mechanisms underlying the effects of CPX on viability of NB cells, microarray analysis was performed in CHP134 control cells and cells treated with 5 ÂµM CPX for 24 hours. Inclusion of both total RNA (reflecting transcriptional status of the cells) and polysomal RNA (approximating the proteomic representation of the cells) provided us with a deeper understanding of changes in the cells upon CPX treatment. Keywords: ciclopirox olamine, iron chelator, neuroblastoma, translatome profiling, transcriptome profiling, polysomal profiling, polysomal RNA, translational control, translational profiling, polysome profiling Microarray analysis was performed to enable a comprehensive view of the changes in the transcriptional and translational status of the cells in response to the treatment with an iron chelator CPX. Polysomal RNA and total RNA were isolated from vehicle-treated CHP134 cells and CHP134 cells treated with 5 ÂµM CPX for 24 hours. Each condition is represented by three biological replicates, i.e. the experiment was repeated starting each time from cells seeding yielding an independent RNA sample. In total, 12 samples corresponding to 4 conditions were subjected to microarray analysis.

Dataset Information

Gene expression profiling of two Alexandrium genotypes as response to waterborne cues from grazing Polykrikos

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets