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Transcription profiling of OsPIL1/DREB1A double-overexpressing Arabidopsis lines.

ABSTRACT: To improve both drought stress tolerance and growth of plants, we generated transgenic Arabidopsis plants that overexpress two transcription factors: DREB1A and OsPIL1. It was reported that DREB1A improves drought stress tolerance in various crops but causes dwarfism, and OsPIL1 enhances cell elongation through activation of cell wall synthesis in rice. We performed microarray experiments using a single overexpressor of OsPIL1 and the double overexpressor of DREB1A and OsPIL1 to study the potential interactions of the gene networks regulated by the two transcription factors in the double overexpressor.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Junya Mizoi

PROVIDER: E-MTAB-4073 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Double overexpression of DREB and PIF transcription factors improves drought stress tolerance and cell elongation in transgenic plants.

Kudo Madoka M Kidokoro Satoshi S Yoshida Takuya T Mizoi Junya J Todaka Daisuke D Fernie Alisdair R AR Shinozaki Kazuo K Yamaguchi-Shinozaki Kazuko K

Plant biotechnology journal 20161114 4

Although a variety of transgenic plants that are tolerant to drought stress have been generated, many of these plants show growth retardation. To improve drought tolerance and plant growth, we applied a gene-stacking approach using two transcription factor genes: DEHYDRATION-RESPONSIVE ELEMENT-BINDING 1A (DREB1A) and rice PHYTOCHROME-INTERACTING FACTOR-LIKE 1 (OsPIL1). The overexpression of DREB1A has been reported to improve drought stress tolerance in various crops, although it also causes a s ...[more]

PMID: 27683092

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