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Transcritpome analysis of Mycobacterium tuberculosis (Mtb) mutants deficient in ergothioneine, or mycothiol production

ABSTRACT: This experiment was performed to determine deferentially regulated genes in the absence of redox buffer ergothioneine (EGT), or mycothiol (MSH). We had used two Mtb mutants namely egtA:Tn and egtD:Tn which are deficient in EGT production ( Transposon Mutant of EGT biosynthesis pathway); In addition, we also used Mtb mshA mutant, which does not produce MSH ( Deletion mutant of Mycothiol biosynthesis ). For microarray analysis, RNA was obtained from exponentially growing (OD 600 nm - 0.6-0.8) CDC1551 (wild type), mshA mutant, egtA:Tn and egtD:Tn strains cultured in 7H9 medium supplemented with ADST (Albumin Dextrose Saline Tyloxapol) using RNApro (MP Biomedicals, USA) as per manufacturer’s instructions. The quality of RNA was examined by running the samples on Experion gene chips (BioRad).

INSTRUMENT(S): Axon 4000B scanner

ORGANISM(S): Mycobacterium tuberculosis CDC1551

SUBMITTER: Vikram Saini 

PROVIDER: E-MTAB-4099 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The mechanisms by which Mycobacterium tuberculosis (Mtb) maintains metabolic equilibrium to survive during infection and upon exposure to antimycobacterial drugs are poorly characterized. Ergothioneine (EGT) and mycothiol (MSH) are the major redox buffers present in Mtb, but the contribution of EGT to Mtb redox homeostasis and virulence remains unknown. We report that Mtb WhiB3, a 4Fe-4S redox sensor protein, regulates EGT production and maintains bioenergetic homeostasis. We show that central c  ...[more]

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