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Cholesterol's effect on M. tuberculosis

ABSTRACT: 2 experiments: (1) Mtb, Tween vs Cholesterol at 3 & 24hrs (2) CDC1551 vs kstR mutant of CDC1551, with and w/o Cholesterol In triplicate, dye flips included.

ORGANISM(S): Mycobacterium tuberculosis

SUBMITTER: Eugenie Dubnau

PROVIDER: E-GEOD-13978 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A thiolase of Mycobacterium tuberculosis is required for virulence and production of androstenedione and androstadienedione from cholesterol.

Nesbitt Natasha M NM Yang Xinxin X Fontán Patricia P Kolesnikova Irina I Smith Issar I Sampson Nicole S NS Dubnau Eugenie E

Infection and immunity 20091012 1

Mycobacterium tuberculosis, the causative agent of tuberculosis, is an intracellular pathogen that shifts to a lipid-based metabolism in the host. Moreover, metabolism of the host lipid cholesterol plays an important role in M. tuberculosis infection. We used transcriptional profiling to identify genes transcriptionally regulated by cholesterol and KstR (Rv3574), a TetR-like repressor. The fadA5 (Rv3546) gene, annotated as a lipid-metabolizing thiolase, the expression of which is upregulated by ...[more]

PMID: 19822655

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