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Unknown,Transcriptomics,Genomics,Proteomics

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Arabidopsis thaliana strain:Columbia 0Transcriptome or Gene expression

ABSTRACT: Mutation of the lignin biosynthetic gene REF8, which encodes p-coumaroylshikimate 3'-hydroxylase, causes widespread changes in gene expression in Arabidopsis thaliana. Disruption of the genes encoding MED5a and MED5b, subunits of the transcriptional coregulatory complex Mediator, rescue the stunted growth of ref8-1 mutants. By comparing gene expression profiles of wild type, ref8-1, med5a/5b, and med5a/5b ref8-1 mutants, we found that the majority of gene expression changes in ref8-1 mutants depend directly or indirectly on MED5a/5b

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Clint Chapple

PROVIDER: E-MTAB-4193 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant.

Bonawitz Nicholas D ND Kim Jeong Im JI Tobimatsu Yuki Y Ciesielski Peter N PN Anderson Nickolas A NA Ximenes Eduardo E Maeda Junko J Ralph John J Donohoe Bryon S BS Ladisch Michael M Chapple Clint C

Nature 20140316 7500

Lignin is a phenylpropanoid-derived heteropolymer important for the strength and rigidity of the plant secondary cell wall. Genetic disruption of lignin biosynthesis has been proposed as a means to improve forage and bioenergy crops, but frequently results in stunted growth and developmental abnormalities, the mechanisms of which are poorly understood. Here we show that the phenotype of a lignin-deficient Arabidopsis mutant is dependent on the transcriptional co-regulatory complex, Mediator. Dis ...[more]

PMID: 24670657

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