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Maize single base resolution methylome in B73 and Mo17 inbred line and their reciprocal hybrid

ABSTRACT: Here we report genome-wide high resolution allele-specific maps of DNA methylation and histone H3 lysine 27 trimethylation (H3K27me3) in maize endosperm. To investigate the allele-specific DNA methylation pattern of maize endosperm on a genome-wide scale, we performed MethylC-seq for shoot, embryo, and endosperm tissue 12 d after pollination (DAP) of inbred B73, and the endosperm tissue 12 DAP of reciprocal crosses B73 Ã Mo17 (BM) and Mo17 Ã B73 (MB). We also performed additional RNA-seq for samples from 12-DAP and 10-DAP endosperm of both reciprocal crosses between inbreds B73 and Mo17

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Zea mays

SUBMITTER: Jin Lai

PROVIDER: E-MTAB-4206 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide high resolution parental-specific DNA and histone methylation maps uncover patterns of imprinting regulation in maize.

Zhang Mei M Xie Shaojun S Dong Xiaomei X Zhao Xin X Zeng Biao B Chen Jian J Li Hui H Yang Weilong W Zhao Hainan H Wang Gaokui G Chen Zongliang Z Sun Silong S Hauck Andrew A Jin Weiwei W Lai Jinsheng J

Genome research 20131016 1

Genetic imprinting is a specific epigenetic phenomenon in which a subset of genes is expressed depending on their parent-of-origin. Two types of chromatin modifications, DNA methylation and histone modification, are generally believed to be involved in the regulation of imprinting. However, the genome-wide correlation between allele-specific chromatin modifications and imprinted gene expression in maize remains elusive. Here we report genome-wide high resolution allele-specific maps of DNA methy ...[more]

PMID: 24131563

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