Unknown,Transcriptomics,Genomics,Proteomics

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Zea mays Transcriptome or Gene expression


ABSTRACT: In this study a transcriptomic approach (RNA-sequencing) was utilized to elucidate molecular responses of maize (Zea mays L.) primary roots of the inbred line B73 to water deficit to gain a better understanding of the mechanisms underlying drought tolerance. Kernels of the maize inbred line B73 were germinated in paper rolls soaked with distilled water until seedlings had a primary root length of 2 to 4 cm. For mild and severe water deficit conditions, seedlings were transferred to PEG8000 solution with water potentials of -0.2 MPa and -0.8 MPa, respectively. Water deficit treatment was applied for 6 h and 24 h. Each treatment was performed in four biological replicates each consisting of 10 roots.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Zea mays

SUBMITTER: Nina Opitz 

PROVIDER: E-MTAB-4325 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptomic complexity in young maize primary roots in response to low water potentials.

Opitz Nina N   Paschold Anja A   Marcon Caroline C   Malik Waqas Ahmed WA   Lanz Christa C   Piepho Hans-Peter HP   Hochholdinger Frank F  

BMC genomics 20140829


<h4>Background</h4>Widespread and more frequently occurring drought conditions are a consequence of global warming and increase the demand for tolerant crop varieties to feed the growing world population. A better understanding of the molecular mechanisms underlying the water deficit response of crops will enable targeted breeding strategies to develop robust cultivars.<h4>Results</h4>In the present study, the transcriptional response of maize (Zea mays L.) primary roots to low water potentials  ...[more]

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