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Tissue specific expression data in Sorghum bicolor BTx623

ABSTRACT: This study used with RNA-Seq to examine the tissue specific expression data within sorghum plants for improving the Sorghum bicolor gene annotation. We examined the RNA from tissues (spikelet, seed and stem) in Sorghum bicolor (BTx623).Total RNAs form each tissues were extracted using SDS/phenol method followed by LiCl purification

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Sorghum bicolor

SUBMITTER: Minami Matsui

PROVIDER: E-MTAB-4273 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MOROKOSHI: transcriptome database in Sorghum bicolor.

Makita Yuko Y Shimada Setsuko S Kawashima Mika M Kondou-Kuriyama Tomoko T Toyoda Tetsuro T Matsui Minami M

Plant & cell physiology 20141209 1

In transcriptome analysis, accurate annotation of each transcriptional unit and its expression profile is essential. A full-length cDNA (FL-cDNA) collection facilitates the refinement of transcriptional annotation, and accurate transcription start sites help to unravel transcriptional regulation. We constructed a normalized FL-cDNA library from eight growth stages of aerial tissues in Sorghum bicolor and isolated 37,607 clones. These clones were Sanger sequenced from the 5' and/or 3' ends and in ...[more]

PMID: 25505007

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