Testing LPS induced gene expression in murine MEFs with different NFkB knockout
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ABSTRACT: NFkB is a family of transcriptional factors that are responsible for inflammatory and immune gene expression. RelA, RelB and cRel are the transactivation domain containing family members. P50 encoded by Nfkb1 is the primary dimerization partner. Many NFkB deficient mice are embryonic lethal. In order to identify NFkB dependent genes, MEF were isolated from Rela-/-cRel-/-, Rela-/-cRel-/-Nfkb1-/- and Rela-/-Relb-/-cRel-/- embryos at E12.5. They were treated with 100ng/ml LPS for 1hr and then profiled gene expression by microarray.
ORGANISM(S): Mus musculus
SUBMITTER: Zhang Cheng
PROVIDER: E-MTAB-4412 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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