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Arabidopsis thaliana strain:Col-0 Transcriptome or Gene expression

ABSTRACT: Arabidopsis thaliana infiltrated with different strains of DC3000 were used to elicit PTI, ETI, or a disease response compared to a mock control. This experiment was carried out in both Col-0 wild-type plants as well as mutants deficient in non-sense mediated decay via disruption of the promoter of upf1 (genotype background upf1-5). To identify differentially expressed and alternatively spliced transcripts elicited by bacterial infection, the NMD-impaired and wild-type host transcriptomes were profiled using RNA-seq. Wild-type and upf1-5 mutant plants were independently challenged with pathogenic (DC3000 COR-) or non-pathogenic P. syringae strains (DC3000 COR- ÎhrpS and DC3000 COR- avrPphB).

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Sergei Filichkin

PROVIDER: E-MTAB-4416 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Environmental stresses modulate abundance and timing of alternatively spliced circadian transcripts in Arabidopsis.

Filichkin Sergei A SA Cumbie Jason S JS Dharmawardhana Palitha P Jaiswal Pankaj P Chang Jeff H JH Palusa Saiprasad G SG Reddy A S N AS Megraw Molly M Mockler Todd C TC

Molecular plant 20150108 2

Environmental stresses profoundly altered accumulation of nonsense mRNAs including intron-retaining (IR) transcripts in Arabidopsis. Temporal patterns of stress-induced IR mRNAs were dissected using both oscillating and non-oscillating transcripts. Broad-range thermal cycles triggered a sharp increase in the long IR CCA1 isoforms and altered their phasing to different times of day. Both abiotic and biotic stresses such as drought or Pseudomonas syringae infection induced a similar increase. Ther ...[more]

PMID: 25680774

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