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Transcription profiling of effected genes by PAD2 and compound 1B8

ABSTRACT: To understand the growth inhibition of peptidylarginine deiminase 2 (PAD2) and small molecule 1B8, colon cancer SW480 cells were treated with compound 1B8 for 24 hours, SW480 cells were transfected with humanPAD2 vector for 24 hours. Cells were harvested and total RNA was extracted using TRIZol method followed by Agilent DNA microarray analysis. To profile the effected genes by compound 1B8, SW480 cells were treated with DMSO (control) and 10uM 1B8 in triplicates in 6-well plate, cells were harvested 24 hours later. To compare the effected genes by compound 1B8 and PAD2 overexpression, SW480 cells were transfected with PAD2 and pIRES2-EGFP empty vector in triplicates in 6 well plates, cells were harvested in 24 hours. All the cells were harvested and total RNA was extracted using TRIZol method followed by Agilent DNA microarray analysis.

INSTRUMENT(S): Scanner G2505B

ORGANISM(S): Homo sapiens

SUBMITTER: Anne Margrete Oyan

PROVIDER: E-MTAB-4452 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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