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Natural variation of gibberellin responses in Arabidopsis thaliana

ABSTRACT: To assess natural variation in the ability to respond to changes in gibberellin metabolism, we examined the effect of the ectopic expression of GA20ox1 in 10 Arabidopsis thaliana accessions. RNA-seq was performed on the sixth leaf, which was micro-dissected (size < 0.25 mm2) at the beginning of the transition from cell proliferation to cell expansion.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Youn-Jeong Nam

PROVIDER: E-MTAB-4508 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Natural Variation of Molecular and Morphological Gibberellin Responses.

Nam Youn-Jeong YJ Herman Dorota D Blomme Jonas J Chae Eunyoung E Kojima Mikiko M Coppens Frederik F Storme Veronique V Van Daele Twiggy T Dhondt Stijn S Sakakibara Hitoshi H Weigel Detlef D Inzé Dirk D Gonzalez Nathalie N

Plant physiology 20161122 1

Although phytohormones such as gibberellins are essential for many conserved aspects of plant physiology and development, plants vary greatly in their responses to these regulatory compounds. Here, we use genetic perturbation of endogenous gibberellin levels to probe the extent of intraspecific variation in gibberellin responses in natural accessions of Arabidopsis (Arabidopsis thaliana). We find that these accessions vary greatly in their ability to buffer the effects of overexpression of GA20o ...[more]

PMID: 27879393

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