Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Hybrid aspen (Populus tremula x tremuloides)1h cytokinin induction and 1h control treatment data.

ABSTRACT: Aim of the project: Genome wide gene expression analysis for cytokinin (100nM 2ip, 20nM NaPi buffer) fast (1h) response genes from 3 months old Hybrid aspen(Populus tremula x tremuloides)apical part (30 cm from tip; diameter:4-5mm) of stem. Stems of two individuals (trees 15 and 16) was cut into 50-100um thick (free hand)cross sections randomly selected stem discs are set to two pools: cytokinin treatment and control treatment. Samples are collected noon time (11:00-11:30). Cytokinin treatment: stem discs (several hundred) are submerged with 100nM 2ip, 20nM NaPi buffer, with modest shaking for 60 min +/- 2 min (time to collect about 30mg stem discs (several dozens) for RNA sample. Control treatment: identical to cytokinin treatment, only without 100nM 2ip.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Populus tremula x Populus tremuloides

SUBMITTER: Olli-Pekka Smolander

PROVIDER: E-MTAB-4635 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Cytokinin and Auxin Display Distinct but Interconnected Distribution and Signaling Profiles to Stimulate Cambial Activity.

Immanen Juha J Nieminen Kaisa K Smolander Olli-Pekka OP Kojima Mikiko M Alonso Serra Juan J Koskinen Patrik P Zhang Jing J Elo Annakaisa A Mähönen Ari Pekka AP Street Nathaniel N Bhalerao Rishikesh P RP Paulin Lars L Auvinen Petri P Sakakibara Hitoshi H Helariutta Ykä Y

Current biology : CB 20160714 15

Despite the crucial roles of phytohormones in plant development, comparison of the exact distribution profiles of different hormones within plant meristems has thus far remained scarce. Vascular cambium, a wide lateral meristem with an extensive developmental zonation, provides an optimal system for hormonal and genetic profiling. By taking advantage of this spatial resolution, we show here that two major phytohormones, cytokinin and auxin, display different yet partially overlapping distributio ...[more]

PMID: 27426519

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Dataset Information

Hybrid aspen (Populus tremula x tremuloides)1h cytokinin induction and 1h control treatment data.

Publications

Cytokinin and Auxin Display Distinct but Interconnected Distribution and Signaling Profiles to Stimulate Cambial Activity.

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