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Ovarian granulosa cell tumor and normal granulosa cells transcriptomes

ABSTRACT: RNA was extracted from normal human granulosa cells from IVF patients (hGC1 and hGC2 samples) and from adult-type ovarian granulosa cell tumor samples (H1, H8, H20, H23, H24, H28, H30, H33, H4, H18) as described in Jamieson et al, 2010. RNA from all samples was linearly amplified using the Whole Transcriptome Amplification kit (Sigma), starting from 300ng of RNA, and with 12 amplifications cycles. cDNA was purified on columns and sent to the Nimblegen platform for hybridization and transcriptional profiling. The FOXL2 locus was gentoyped in tumor samples, and all samples were found positive for the recurrent somatic mutation p.Cys134Trp which is present in >95% of adult-type ovarian granulosa cell tumors (Shah et al, 2009).

ORGANISM(S): Homo sapiens

DISEASE(S): ovarian granulosa cell tumor, adult type

SUBMITTER: BM-irM-inice Benayoun

PROVIDER: E-MTAB-483 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Adult ovarian granulosa cell tumor transcriptomics: prevalence of FOXL2 target genes misregulation gives insights into the pathogenic mechanism of the p.Cys134Trp somatic mutation.

Benayoun B A BA Anttonen M M L'Hôte D D Bailly-Bechet M M Andersson N N Heikinheimo M M Veitia R A RA

Oncogene 20120716 22

Ovarian granulosa cell tumors (OGCT) are the most frequent kind of sex cord-stromal tumors, and represent ∼2-5% of all ovarian malignancies. OGCTs exist as two entities, juvenile and adult types, with specific clinical and pathological characteristics. The molecular pathogenesis of these tumors has just begun to be unraveled. Indeed, recent studies have indicated that mutation and/or misregulation of the key ovarian transcription factor FOXL2 has a role in OGCT formation, although the mechanisms ...[more]

PMID: 22797072

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