Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of immature and LPS-matured dendritic cells, sorted for DC-S and DC-L phenotypes


ABSTRACT: We have described two human, monocyte-derived dendritic cells (DC), which we have termed DC-S (small) and DC-L (large). Human dendritic cells are significantly less well known that murine ones, yet they are a crucial player in the immune system, especially when considering options for immunotherapies. Specifically, monocyte-derived dendritic cells, which are the most amenable for clinically relevant work, are considered by many as only reactive, inflammatory cells. In my work I showed that in fact they consist of two subpopulations, with diverging characteristics: one is steady state-like and more tolerizing in its phenotype (DC-S), and the other is more “dangerous”, with an inflammatory phenotype (DC-L). This has important implications for the immunobiology of dendritic cells and also for their use in clinical applications.
As part of our effort to better characterize them, while immature (day 6 of culture) we sorted them into DC-S and DC-L, replated them with or without LPS for 24 hours, and then extracted the RNA. 3 different experiments were pooled together for the RNA samples. The source of the cells is from random donor blood bank buffy coat preparations - we don't know their identities or demographics. The DCs were prepared as described in our manuscript; briefly, monocytes were isolated from buffy coats using CD14 magnetic beads and cultured for 6 days with 1% autologous plasma, IL4 and GCSF. At day 6 we sorted them as described above.

ORGANISM(S): Homo sapiens

SUBMITTER: Uriel Trahtemberg 

PROVIDER: E-MTAB-5075 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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