Dataset Information
Microarray profiling of polyploid macrophage progeny
Ontology highlight
ABSTRACT:
-Day 0: After bone marrow culture for 5 days with M-CSF, adherent cells were collected and analyzed.
-Day 3, M-CSF: Bone marrow was cultured for 5 days with M-CSF, adherent cells were then collected, re-plated and treated with M-CSF for 3 days. Adherent cells were collected and analyzed.
-Day 3, FSL-1: Bone marrow was cultured for 5 days with M-CSF, adherent cells were then collected, re-plated and treated with M-CSF and FSL-1 for 3 days. Adherent cells were collected and analyzed.
-Day 6, M-CSF: Bone marrow was cultured for 5 days with M-CSF, adherent cells were then collected, re-plated and treated with M-CSF for 6 days. Adherent cells were collected and analyzed.
-Day 6, FSL-1: Bone marrow was cultured for 5 days with M-CSF, adherent cells were then collected, re-plated and treated with M-CSF and FSL-1 for 6 days. Adherent cells, containing polyploid macrophages, were collected and analyzed.
ORGANISM(S): Mus musculus
SUBMITTER: Antigoni Triantafyllopoulou
PROVIDER: E-MTAB-5085 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
DNA Damage Signaling Instructs Polyploid Macrophage Fate in Granulomas.
Cell 20161027 5
Granulomas are immune cell aggregates formed in response to persistent inflammatory stimuli. Granuloma macrophage subsets are diverse and carry varying copy numbers of their genomic information. The molecular programs that control the differentiation of such macrophage populations in response to a chronic stimulus, though critical for disease outcome, have not been defined. Here, we delineate a macrophage differentiation pathway by which a persistent Toll-like receptor (TLR) 2 signal instructs p ...[more]
