RNAseq analysis of GM-CSF and M-CSF polarized bone marrow derived macrophages cultured on TC or noTC dishes
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ABSTRACT: Bone marrow cells were isolated, primed with M-CSF (M BMDM) or GM-CSF (GM BMDM) and cultured on tissue culture dishes (TC) or low adherent dishes (noTC) for 7 days. BMDMs were treated with LPS (100 ng/ml) for 3 hours. The difference between TC- and noTC-cultured BMDMs were analyzed to describe the phenotye and function of each population. Moreover, the influence of LPS treatment on GM-BMDMs and M-BMDMs were analyzed.
ORGANISM(S): Mus musculus
PROVIDER: GSE198821 | GEO | 2022/06/10
REPOSITORIES: GEO
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