Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression profiling of 3 and 24 months mouse skeletal muscle (RNA-seq)

ABSTRACT: Aging in multicellular organisms is characterized by gradual decline of organ functionality. To study the aging process, we used mRNA sequencing (mRNA-seq) to identify gene expression changes during aging in healthy mice. Skeletal muscle tissues of wild-type mice at 3 months and 24 months of age were collected and mRNA-seq libraries were prepared and sequenced on a HiSeq2500 by single-end sequencing with 100 bp read length. Analysis of the expression profiles of aged skeletal muscle tissues showed decreased mRNA levels of genes function in lipid metabolism, peptidase activity and response to stimulus.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: I-Hsuan Lin

PROVIDER: E-MTAB-5176 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Skeletal muscle in aged mice reveals extensive transformation of muscle gene expression.

Lin I-Hsuan IH Chang Junn-Liang JL Hua Kate K Huang Wan-Chen WC Hsu Ming-Ta MT Chen Yi-Fan YF

BMC genetics 20180808 1

<h4>Background</h4>Aging leads to decreased skeletal muscle function in mammals and is associated with a progressive loss of muscle mass, quality and strength. Age-related muscle loss (sarcopenia) is an important health problem associated with the aged population.<h4>Results</h4>We investigated the alteration of genome-wide transcription in mouse skeletal muscle tissue (rectus femoris muscle) during aging using a high-throughput sequencing technique. Analysis revealed significant transcriptional ...[more]

PMID: 30089464

Publication: 1/2

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Project description:Maternal obesity can program metabolic syndrome in offspring but the mechanisms are not well characterized. Moreover, the consequences of maternal overnutrition in the absence of frank obesity remain poorly understood. This study aimed to determine the effects of maternal consumption of a high fat-sucrose diet on the skeletal muscle metabolic and transcriptional profiles of adult offspring. Female Sprague Dawley rats were fed either a diet rich in saturated fat and sucrose (HFD, 23.5% fat, 20% sucrose wt/wt) or a standard chow diet (NFD, 7% fat, 10% sucrose w/w) for the 3 weeks prior to mating and throughout pregnancy and lactation. Although maternal weights were not different between groups at conception or weaning, HFD dams were ~22% heavier than chow fed dams from mid-pregnancy until 4 days post-partum. Adult male offspring of HFD dams were not heavier than controls but demonstrated features of insulin resistance including elevated plasma insulin concentration (+40%, P<0.05). Next Generation mRNA Sequencing was used to identify differentially expressed genes in the soleus muscle of offspring, and Gene Set Enrichment Analysis (GSEA) to detect coordinated changes that are characteristic of a biological function. GSEA identified 15 pathways enriched for up-regulated genes, including cytokine signaling (P<0.005), starch and sucrose metabolism (P<0.017), and inflammatory response (P<0.024). A further 8 pathways were significantly enriched for down-regulated genes including oxidative phosphorylation (P<0.004) and electron transport (P<0.022). Western blots confirmed a ~60% reduction in the phosphorylation of the insulin signaling protein Akt (P<0.05) and ~70% reduction in mitochondrial complexes II (P<0.05) and V expression (P<0.05). On a normal diet, offspring of HFD dams developed an insulin resistant phenotype, with transcriptional evidence of muscle cytokine activation, inflammation and mitochondrial dysfunction. These data indicate that maternal overnutrition, even in the absence of pre-pregnancy obesity can promote metabolic dysregulation and predispose offspring to type 2 diabetes. Messenger RNA profile of skeletal muscle of male offspring from female Sprague Dawley rats fed either a diet rich in saturated fat and sucrose (HFD, 23.5% fat, 20% sucrose wt/wt) or a standard chow diet (NFD, 7% fat, 10% sucrose w/w) for the 3 weeks prior to mating and throughout pregnancy and lactation. There were 5 HFD samples compared to 6 NFD control samples.

Dataset Information

Expression profiling of 3 and 24 months mouse skeletal muscle (RNA-seq)

Publications

Skeletal muscle in aged mice reveals extensive transformation of muscle gene expression.

Similar Datasets

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