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RNA-Seq of steady-state and LPS stimulated splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice

ABSTRACT: The transcriptome of splenic classical dendritic cells (cDCs) (CD3- CD19- CD45+ MHC-II+ CD11c+) was compared in Wild Type (WT) mice, Germ Free (GF) mice and mice deficient for the Ifnar1 gene (IFNAR). Cells were isolated and sorted by flow cytometry to high purity. The cells were stimulated ex vivo with PBS or LPS. The results show remarkable similarities between the transcriptome of splenic cDCs in GF and IFNAR mice.

INSTRUMENT(S): Illumina HiSeq 2500, BD ARIA II Cell Sorter

ORGANISM(S): Mus musculus

SUBMITTER: Leif Rogell

PROVIDER: E-MTAB-5190 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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