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ChIP-Seq (H3K4me3, H3K27me3) of steady-state splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice

ABSTRACT: The epigenetic state of splenic cDCs from Wild Type (WT) mice, Germ Free (GF) and mice deficient for the Ifnar1 gene (IFNAR) was characterized by analyzing the occupancy of a major activating histone mark (H3K4me3) and a major repressive histone mark (H3K27me3). Splenic cDCs (CD3- CD19- CD45+ MHC-II+ CD11c+) were sorted to high purity by flow cytometry and subject to chromatin immunoprecipitation.

INSTRUMENT(S): Illumina HiSeq 2500, BD ARIA II Cell Sorter

ORGANISM(S): Mus musculus

SUBMITTER: Andreas Diefenbach

PROVIDER: E-MTAB-5234 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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