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Effect of silencing DLC1 (Deleted in Liver Cancer 1) in Human Umbilical Vein Endothelial Cells (HUVEC)

ABSTRACT: We observed that silencing DLC1 in HUVEC made them more resilient to certain types of cell death. We wondered what type of mechanism is protecting the cells from death induced after long term confluence culture. HUVEC were transduced with the control (pLKO) or silencing vectors shDLC1#3 or #7 (TRCN0000047823, TRCN0000047827; all from Sigma). The cells were grown for different times in optimal culture conditions, and then were grown to full confluence (to a time where cell death was not immediately apparent) and RNA was extracted and studied by microarray

ORGANISM(S): Homo sapiens

SUBMITTER: Giovanna Tosato

PROVIDER: E-MTAB-5263 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Effects of DLC1 Deficiency on Endothelial Cell Contact Growth Inhibition and Angiosarcoma Progression.

Sánchez-Martín David D Otsuka Atsushi A Kabashima Kenji K Ha Taekyu T Wang Dunrui D Qian Xiaolan X Lowy Douglas R DR Tosato Giovanna G

Journal of the National Cancer Institute 20180401 4

<h4>Background</h4>Deleted in Liver Cancer 1 (DLC1) is a tumor suppressor gene frequently deleted in cancer. However, DLC1 is not known to be deleted in angiosarcoma, an aggressive malignancy of endothelial cell derivation. Additionally, the physiologic functions of DLC1 protein in endothelial cells are poorly defined.<h4>Methods</h4>We investigated the effects of shRNA-induced DLC1 depletion in endothelial cells. Cell growth was measured by 3H thymidine incorporation, IncuCyte imaging, and popu ...[more]

PMID: 29202196

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