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Antibody repertoire RNA-seq throughout key stages of mouse B-cell development (pre-B-cells, naive B cells, plasma cells)

ABSTRACT: We sought to investigate the extent of genetic and antigen-driven predetermination of antibody repertoires throughout B-cell development (pre-B cells, naive B cells, plasma cells). To this end, we used (i) an inbred model organism (mouse, C57BL/6J) to quantify genetic predetermination and (ii) cohort-stratification (4 cohorts: 1 untreated, 3 antigen-immunized) to quantify antigen-driven predetermination. To ensure generalizability of our research, we additionally sequenced naive B cells from Balb/c and pet shop mice.

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Mus musculus

SUBMITTER: Victor Greiff

PROVIDER: E-MTAB-5349 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Comprehensive evaluation and optimization of amplicon library preparation methods for high-throughput antibody sequencing.

Menzel Ulrike U Greiff Victor V Khan Tarik A TA Haessler Ulrike U Hellmann Ina I Friedensohn Simon S Cook Skylar C SC Pogson Mark M Reddy Sai T ST

PloS one 20140508 5

High-throughput sequencing (HTS) of antibody repertoire libraries has become a powerful tool in the field of systems immunology. However, numerous sources of bias in HTS workflows may affect the obtained antibody repertoire data. A crucial step in antibody library preparation is the addition of short platform-specific nucleotide adapter sequences. As of yet, the impact of the method of adapter addition on experimental library preparation and the resulting antibody repertoire HTS datasets has not ...[more]

PMID: 24809667

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