Microarray of iPSC-derived macrophages generated in a bioreactor process compared to PBMC-derived macrophages and exposed to P. aeruginosa
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ABSTRACT: Human iPSC-Mac or PBMC-mac were seeded on 24-well plates (500.000cells/well) and cultured over night. On the next day, cells were washed three times with PBS. Subsequently, P. aeruginosa laboratory strain PAO1 in RPMI medium without antibiotics (MOI10) was centrifugated on the cells (600xg) and incubated at 37°C. Cells with medium only served as non-infected controls. After 1h cells were de-attached, washed and resuspended in RNA lysis buffer. RNA isolation was performed with RNAeasy micro Kit (Qiagen), according to manufactures instructions. Human iPSC samples were obtained after sorting of iPSC for TRA-1-60+ to separate iPSCs from feeder cells.
ORGANISM(S): Homo sapiens
SUBMITTER: Mania Ackermann
PROVIDER: E-MTAB-5436 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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