Dataset Information

Systematic identification of hypothetical bacteriophage proteins targeting key protein complexes of Pseudomonas aeruginosa

ABSTRACT: Addressing the functionality of predicted genes remains an enormous challenge in the post-genomic era. A prime example of genes lacking functional assignments are the poorly conserved, early expressed genes of lytic bacteriophages, whose products are involved in the subversion of the host metabolism. In this study, we focused on the composition of important macromolecular complexes of Pseudomonas aeruginosa involved in transcription, DNA replication, fatty acid biosynthesis, RNA regulation, energy metabolism and cell division, during infection with members of seven distinct clades of lytic phages. Using affinity purifications of these host protein complexes coupled to mass spectrometric analyses, 37 host complex-associated phage proteins could be identified. Importantly, eight of these show an inhibitory effect on bacterial growth upon episomal expression, suggesting that these phage proteins are potentially involved in hijacking the host complexes. Using complementary protein-protein interaction assays, we further mapped the inhibitory interaction of gp12 of phage 14-1 to the α subunit of the RNA polymerase. Together, our data demonstrate the powerful use of interactomics to unravel the biological role of hypothetical phage proteins, which constitute an enormous untapped source of novel antibacterial proteins.

INSTRUMENT(S): LTQ Orbitrap Velos, LTQ

ORGANISM(S): Pseudomonas Aeruginosa Pao1

SUBMITTER: Jean-Paul Noben

LAB HEAD: Prof Rob Lavigne

PROVIDER: PXD001199 | Pride | 2014-09-09

REPOSITORIES: Pride

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Systematic identification of hypothetical bacteriophage proteins targeting key protein complexes of Pseudomonas aeruginosa.

Van den Bossche An A Ceyssens Pieter-Jan PJ De Smet Jeroen J Hendrix Hanne H Bellon Hannelore H Leimer Nadja N Wagemans Jeroen J Delattre Anne-Sophie AS Cenens William W Aertsen Abram A Landuyt Bart B Minakhin Leonid L Severinov Konstantin K Noben Jean-Paul JP Lavigne Rob R

Journal of proteome research 20140915 10

Addressing the functionality of predicted genes remains an enormous challenge in the postgenomic era. A prime example of genes lacking functional assignments are the poorly conserved, early expressed genes of lytic bacteriophages, whose products are involved in the subversion of the host metabolism. In this study, we focused on the composition of important macromolecular complexes of Pseudomonas aeruginosa involved in transcription, DNA replication, fatty acid biosynthesis, RNA regulation, energ ...[more]

PMID: 25185497

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			Action	DRS
	110802_GlcB_PEV2.sf3	Other
	110802_GlcB_phiKZ.sf3	Other
	110822_GlcB_1411.sf3	Other
	110822_GlcB_LUZ24.sf3	Other
	110822_GlcB_YuA.sf3	Other