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Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of the human breast cancer ERÎ±-suppressed MCF-7(MCF-7/SP10+) cells and of their internal control MCF-7 (MCF-7/C) cells

ABSTRACT: Estrogens receptor a (ERÎ±) is essential for breast tumors,since about seventy percent of breast cancers are detected as ERÎ± positive.Recent studies suggest that ERÎ± is related with the epithelial cell morphology. Recently, it has demonstrated that the suppression of ERÎ± induced epithelial-mesenchymal transition (EMT) in the MCF-7 breast cacner cells. Interestingly, the loss of ERa resulted in strong differences on the gene expression profile of a variety of genes. Therefore, the aim of the RNA-seq is to elucidate the effect of the silencing of ERÎ± on the mRNA levels of a larger variety of genes, thus revealing possible target genes which may be implicated on the aggressive phenotype and behavior of the ERÎ±-suppresed MCF-7/SP10+ breast cancer cells. For this reason total RNA from both MCF-7/SP10+ cells and of their internal control MCF-7/C cells was extracted in 3 biological replicates and 3 technical replicates.

INSTRUMENT(S): Ion Torrent Proton

ORGANISM(S): Homo sapiens

SUBMITTER: Aristidis Moustakas

PROVIDER: E-MTAB-5537 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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