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Lineage-dependent spatial and functional organisation of the mammalian enteric nervous system

ABSTRACT: Single-cell transcriptomic analysis to delineate key regulators of neurogenic commitment in the enteric nervous system. To resolve the molecular profiles of ENCCs and identify transcriptional signatures of lineage restriction and differentiation, we analyzed their transcriptomes by single-cell RNA-sequencing (scRNA-seq). E12.0 Sox10::CreERT2;Rosa26tdTomato (SER93|tdT) embryos were exposed to a single dose of TM (100g/g of dam body weight) and tdT+ gut cells were isolated 20-24 hours later by flow cytometry.

INSTRUMENT(S): Linux Beowulf cluster, Linux computer, Illumina HiSeq 4000, FACSAria Fusion

ORGANISM(S): Mus musculus

SUBMITTER: Reena Lasrado

PROVIDER: E-MTAB-5553 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Lineage-dependent spatial and functional organization of the mammalian enteric nervous system.

Lasrado Reena R Boesmans Werend W Kleinjung Jens J Pin Carmen C Bell Donald D Bhaw Leena L McCallum Sarah S Zong Hui H Luo Liqun L Clevers Hans H Vanden Berghe Pieter P Pachnis Vassilis V

Science (New York, N.Y.) 20170501 6339

The enteric nervous system (ENS) is essential for digestive function and gut homeostasis. Here we show that the amorphous neuroglia networks of the mouse ENS are composed of overlapping clonal units founded by postmigratory neural crest-derived progenitors. The spatial configuration of ENS clones depends on proliferation-driven local interactions of ENS progenitors with lineally unrelated neuroectodermal cells, the ordered colonization of the serosa-mucosa axis by clonal descendants, and gut exp ...[more]

PMID: 28522527

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