Unknown,Transcriptomics,Genomics,Proteomics

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Microarray analysis of gene expression in Phytophthora capsici over-expressing an NMRA-like protein (PcNMRAL1, Phyca11_505845) during a time course infection assay of tomato (Solanum lycopersicum) leaves

ABSTRACT: Phytophthora capsici is a broad host range hemi-biotrophic pathogen which infects a wide range of agriculturally important crop plants. Infection with P. capsici is characterised by an early biotrophic phase which involves the formation of haustoria, followed by a necrotrophic phase later during infection. Previous data from our lab (Experiment E-MTAB-1295) revealed distinct transcriptional changes associated with biotrophy and necrotrophy, suggesting tight control and regulation of gene expression in P. capsici during the infection process, involving transcription factors and transcriptional regulators. A gene encoding for an NMRA-like protein (PcNMRAL1, Phyca11_505845) was identified as co-expressed with the biotrophy marker gene PcHmp1 encoding for a haustorial membrane protein. As this gene was found to be expressed during infection, and NMRA-like proteins are known to act as transcriptional regulators in fungi, this experiment aimed to investigate the effect of over-expression of PcNmrAL1 on gene expression in P. capsici (LT6535) during the early phase of infection (up to 24 hours post inoculation) on tomato leaves. P. capsici expressing the tdTomato fluorescence protein was used as a control strain. The results suggest PcNMRAL1 regulates the infection process by induction of biotrophy-related genes and suppression of necrotrophy-related genes. Tissue was sampled at 8, 16 and 24 hours post inoculation (hpi) for in planta stages, and at 24 hours for the in vitro mycelial stage.

ORGANISM(S): Phytophthora capsici

SUBMITTER: Edgar Huitema

PROVIDER: E-MTAB-5620 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Microarray analysis of gene expression in Phytophthora capsici over-expressing an NMRA-like protein (PcNMRAL1, Phyca11_505845) during a time course infection assay of tomato (Solanum lycopersicum) leaves

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