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RNA-seq of chemically reset human naive pluripotent stem cells

ABSTRACT: A simple method is presented to reset human pluripotent cells to a naive state via transient histone deacetylase inhibition and maintenance in chemically-defined naive stem cell culture media. Cells can be reset without transgenes and expanded continuously either on feeders or alternative substrates in feeder-free conditions. Multiple cell lines of varying origin were reset and characterised in parallel with conventionally cultured counterparts.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Homo sapiens

SUBMITTER: Paul Bertone

PROVIDER: E-MTAB-5674 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Epigenetic resetting of human pluripotency.

Guo Ge G von Meyenn Ferdinand F Rostovskaya Maria M Clarke James J Dietmann Sabine S Baker Duncan D Sahakyan Anna A Myers Samuel S Bertone Paul P Reik Wolf W Plath Kathrin K Smith Austin A

Development (Cambridge, England) 20170801 15

Much attention has focussed on the conversion of human pluripotent stem cells (PSCs) to a more naïve developmental status. Here we provide a method for resetting via transient histone deacetylase inhibition. The protocol is effective across multiple PSC lines and can proceed without karyotype change. Reset cells can be expanded without feeders with a doubling time of around 24 h. WNT inhibition stabilises the resetting process. The transcriptome of reset cells diverges markedly from that of prim ...[more]

PMID: 28765214

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