Transcriptional profiling by array of mouse hearts from animals with heterozygous truncation of Igf1 gene (HZ), or HZ treated with low doses of IGF-1 (substitutive therapy) against wild-type control
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ABSTRACT: 'This study investigates the effects produced by IGF-1 partial deficiency and if those effects were restored with the hormone''s substitutive therapy. Partial IGF-1 deficiency is achieved by heterozygous animals for a truncated gene of IGF-1 (Hz Igf1+/-), supplied by The Jackson Laboratories, which show a 60-75% reduction in circulating IGF-1 levels. The mice were a cross of 129SV Igf1+/- mice with non-consanguineous MF-1 mice to avoid consanguinity. Animals are treated for 10 days 2ug/100g bw/day with rhIGF-1 in succinate vehicle. After the 10 days of treatment, the animals were sacrificed, hearts were extracted, stored in RNAlater and frozen. On the day of the experiment, hearts were thawed, homogenised and subsequently processed as the RNA extraction kit instructions indicate (RNAqueousH-Micro Kit, Ambion, USA).'
ORGANISM(S): Mus musculus
SUBMITTER: Gabriel Aguirre
PROVIDER: E-MTAB-5791 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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