Project description:CHX is an inhibitor of translation elongation often used in ribosome profiling experiments. There is evidence that CHX treatment of cells may cause artefacts in the distribution of ribosomes on mRNAs. We investigate this possibility in S. pombe by performing ribosome profiling in the presence and absence of this drug.
Project description:Translation complexes are stabilised in vivo using formaldehyde. Cells are lysed, and cell extracts are treated with RNase I to produce protected RNA fragments (FPs or footprints). Extracts are then run through sucrose gradients to separate small ribosomal subunits and full ribosomes. FPs are isolated from each of these fractions and analysed by sequencing. Note this is a modified version of ribo-seq (a.k.a. ribosome profiling)
Project description:Ribosome profiling was used to investigate how Schizosaccharomcyes pombe cells modulate gene expression in response to hydrogen peroxide
Project description:Ribosome profiling was used to investigate how Schizosaccharomcyes pombe cells modulate gene expression in response to cadmium exposure
Project description:Ribosome profiling was used to investigate how Schizosaccharomcyes pombe cells modulate gene expression in response to osmotic shock
Project description:Ribosome profiling was used to investigate how Schizosaccharomcyes pombe cells modulate gene expression in response to methyl methanesulfonate (MMS)