Project description:We applied in parallel RNA-Seq and Ribosome-profiling to vegetatively growing S. pombe cells. Two independent biological repeats were performed.
Project description:We applied in parallel RNA-Seq and Ribosome-profiling to S. pombe pat1 diploids undergoing meiosis and sporulation in a synchronous manner
Project description:We applied in parallel RNA-Seq and Ribosome-profiling to S. pombe pat1 diploids undergoing meiosis and sporulation in a synchronous manner
Project description:To estimate mRNA steady-state levels we used RNA extracted from logarithmically growing fisson yeast cells on Affymetrix Yeast 2.0 Genechip arrays. The signal intensities from two independent biological repeats were averaged, resulting in measurements for 4818 out of 4962 nuclear protein-coding genes.
Project description:We estimated global mRNA half-lives by blocking transcription and measuring mRNA levels at different times after transcriptional shut-off.
Project description:Data on absolute molecule numbers are rare but will empower the modeling, understanding and comparison of cellular functions and biological systems. We quantified transcriptomes and proteomes in fission yeast during both proliferation and quiescence. This rich resource provides the first comprehensive reference for all RNA and most protein concentrations in a eukaryote under key physiological conditions. This integrated dataset will support quantitative biology and afford unique biological insights into cell regulation. While mRNAs are typically expressed in a narrow range above 1 copy/cell, most long non-coding RNAs, except for a specific subset, are strongly repressed below 1 copy/cell. Cell cycle-regulated transcription tunes mRNA numbers to phase-specific requirements but can also lead to switch-like expression. Proteins greatly exceed mRNAs in abundance and dynamic range, and their numbers scales with functional demands. Upon transition to quiescence, the proteome composition changes substantially but, in stark contrast to mRNAs, proteins do not uniformly decrease but scale with cell volume.