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Transcriptional profiling of the developmental gene whiH deletion mutant of Streptomyces venezuelae ATCC 10712 at 7 time points from 8 to 20 hours of growth.

ABSTRACT: WhiH is a transcription factor found in Streptomyces species. It has a role in sporulation in these bacteria. Although mutants in whiH are able to form aerial mycelia, these fail to differentiate into spores. The aim of this transcription profiling experiment was to measure genome wide transcript levels in the wild type and the whiH deletion mutant at 7 time points from 8 to 20 hours during the growth cycle of Streptomyces venezuelae.

ORGANISM(S): Streptomyces venezuelae

SUBMITTER: Maureen Bibb

PROVIDER: E-MTAB-5853 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling by array of Streptomyces venezuelae wild type and bldN mutant strains

Project description:Transcription profiling by array of Streptomyces venezuelae wild type and bldN mutant strains during a time course from 8 to 20 hours, sampling at 2 hour intervals.

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Identification by ChIP-Seq of the binding sites of the developmental protein WhiH in the genome of Streptomyces venezuelae

Project description:The whiH gene is required for the differentiation of aerial hyphae into spores in Streptomyces species. It is a predicted member of the GntR family of transcription factors and has been shown to bind specifically to a sequence in its own promoter. This ChIP-Seq experiment was carried out to determine all the binding sites whiH binds to in the genome of Streptomyces venezuelae. A whiH deletion strain was made and a FLAG tagged whiH protein was expressed in it from a genome-integrated plasmid. Then anti-FLAG antibodies were used for chromatin immunoprecipitation followed by high throughput sequencing. The wild type Streptomyces venezuelae strain (ATCC 10712) was used as a negative control. For both the FLAG-WhiH strain and the WT strain, non-immunoprecipitated (total) DNA was also sequenced to arrive at a background enrichment which could be subtracted from the enrichment in the immunoprecipated sample.

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Differential gene expression analysis by RNA-Seq for the bldC deletion strain and the wild type of Streptomyces venezuelae strain NRRL B-65442 after 10 and 14 hours of culture

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Transcriptional profiling of the developmental gene whiA deletion mutant of Streptomyces venezuelae ATCC 10712 at 7 time points from 8 to 20 hours of growth.

Project description:WhiA is an unusual transcriptional regulator found in Streptomyces species related to eukaryotic homing endonucleases. It plays a central role in sporulation in these bacteria. The aim of this transcription profiling experiment was to measure genome wide transcript levels in the wild type and the whiA deletion mutant at 7 time points from 8 to 20 hours during the growth cycle of Streptomyces venezuelae.

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Transcriptional profiling of the developmental gene whiG deletion mutant of Streptomyces venezuelae ATCC 10712 at 7 time points from 8 to 20 hours of growth.

Project description:WhiG is a sigma factor found in Streptomyces species. It has a central role in sporulation in these bacteria. Although mutants in whiG are able to form aerial mycelia, these fail to differentiate into spores. The aim of this transcription profiling experiment was to measure genome wide transcript levels in the wild type and the whiG deletion mutant at 7 time points from 8 to 20 hours during the growth cycle of Streptomyces venezuelae.

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Transcriptional profiling of the developmental gene whiB deletion mutant of Streptomyces venezuelae ATCC 10712 at 7 time points from 8 to 20 hours of growth

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Genes required for aerial growth, cell division, and chromosome segregation are targets of WhiA before sporulation in Streptomyces venezuelae.

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RNA-Seq of Streptomyces venezuelae grown overnight (14 hrs) with Mitomycin-C against untreated controls.

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2022-07-06 | E-MTAB-11556 | biostudies-arrayexpress

RNA-Seq of Streptomyces venezuelae to compare global gene expression between the wild-type and BldB deletion strains after 10, 14 and 22 hours of growth.

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Identification by ChIP-Seq of the binding sites of the sigma factor WhiG in the genome of Streptomyces venezuelae

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