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RNA-seq of coding RNA from USP14 knockout human haploid (HAP1) cells

ABSTRACT: We performed a genome-scale screen for suppressors of interferon stimulated gene (ISG) expression in human haploid cells (HAP1). Ubiquitin specific peptidase 14 (USP14) was a significant hit. In order to validate USP14 as a regulator of ISG expression, we created knockouts of USP14 in HAP1 cells using CRISPR-Cas9 and performed RNA-seq on coding RNA from USP14 KO and WT cells. This data was used to determine if ISGs were upregulated in USP14 KO HAP1 cells.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Jan Carette

PROVIDER: E-MTAB-5865 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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